蔡洪生,魏学娜,高艺嘉,苏浩洋,王杰,郭长虹

• 1:哈尔滨市农业科学院生物中心
• 2:哈尔滨师范大学生命科学与技术学院黑龙江省分子细胞遗传与遗传育种重点实验室

摘要(Abstract)：

在全基因组水平上对蒺藜苜蓿(Medicago truncatula)PR-1基因家族进行了系统鉴定、结构分析与表达谱解析，旨在探讨该家族基因在蒺藜苜蓿中的潜在功能。共鉴定出20个PR-1基因，命名为MtPR-1。结构域分析显示，这些基因编码的蛋白长度各异，但均包含保守的CAP (Cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins)结构域及CBM (Caveolin-binding motif)。染色体定位分析表明，蒺藜苜蓿PR-1基因在染色体上分布不均，其中大部分成员(12/20)聚集于2号染色体。启动子区顺式作用元件分析揭示，MtPR-1基因上游存在多种参与植物代谢调控的关键调控元件。基于涵盖不同器官组织、非生物胁迫及生物胁迫条件下共计431个(若考虑生物学重复则为1 374个)转录组样本的表达谱分析显示，大多数MtPR-1基因在多种条件下呈现差异表达模式。本研究为深入理解PR-1蛋白的生物学功能提供了重要的基础数据，也为培育具有多胁迫抗性的蒺藜苜蓿新品种提供了潜在的候选基因资源。

关键词(KeyWords)： 蒺藜苜蓿;PR-1基因;结构分析;生物和非生物胁迫

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省博士后科学基金资助项目(LBH-Z20160);; 黑龙江省自然科学基金资助项目(YQ2022C025)

作者(Author): 蔡洪生,魏学娜,高艺嘉,苏浩洋,王杰,郭长虹

DOI: 10.13482/j.issn1001-7011.2026.03.102

参考文献(References)：

• [1] BREEN S,WILLIAMS S J,OUTRAM M,et al.Emerging insights into the functions of pathogenesis-related protein 1 [J].Trends in Plant Science,2017,22(10):871-879.
• [2] SELS J,MATHYS J,DE CONINCK B M,et al.Plant pathogenesis-related (PR) proteins:a focus on PR peptides [J].Plant Physiology and Biochemistry,2008,46(11):941-950.
• [3] VAN LOON L C,GERRITSEN Y A M,RITTER C E.Identification,purification,and characterization of pathogenesis-related proteins from virus-infected Samsun NN tobacco leaves [J].Plant Molecular Biology,1987,9(6):593-609.
• [4] ALEXANDER D,GOODMAN R M,GUT-RELLA M,et al.Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a [J].Proceedings of the National Academy of Sciences of the United States of America,1993,90(15):7327-7331.
• [5] GIBBS G M,ROELANTS K,O’BRYAN M K.The CAP superfamily:cysteine-rich secretory proteins,antigen 5,and pathogenesis-related 1 proteins-roles in reproduction,cancer,and immune defense [J].Endocrine Reviews,2008,29(7):865-897.
• [6] TELLIS M,MATHUR M,GURJAR G,et al.Identifification and functionality prediction of pathogenesis-related protein 1 from legume family [J].Proteins:Structure,Function,and Bioinformatics,2017,85:2066-2080.
• [7] DARWICHE R,EL ATAB O,COTTIER S,et al.The function of yeast cap family proteins in lipid export,mating,and pathogen defense [J].FEBS Letters,2018,592:1304-1311.
• [8] CHEN Y L,LEE C Y,CHENG K T,et al.Quantitative peptidomics study reveals that a wound-induced peptide from PR-1 regulates immune signaling in tomato [J].The Plant Cell,2014,26(10):4135-4148.
• [9] BREEN S,WILLIAMS S J,OUTRAM M,et al.Emerging insights into the functions of pathogenesis-related protein 1 [J].Trends in Plant Science,2017,22(10):871-879.
• [10] KOTHARI K S,DANSANA P K,GIRI J,et al.Rice stress associated protein 1 (OsSAP1) interacts with aminotransferase (OsAMTR1) and pathogenesis-related 1a protein (OsSCP) and regulates abiotic stress responses [J].Frontiers in Plant Science,2016,7:1057.
• [11] SEO P J,LEE A K,XIANG F,et al.Molecular and functional profiling of Arabidopsis pathogenesis-related genes:insights into their roles in salt response of seed germination [J].Plant and Cell Physiology,2008,49(3):334-344.
• [12] LI Y,HOSSAIN M S,LIBAULT M.Single-cell omics in legumes:research trends and applications [J].Plants,2025,14(23):3615.
• [13] 周玲芳，尚骁尧，张铁军，等.蒺藜苜蓿(Medicago truncatula)叶片衰老的转录组分析 [J].草地学报，2021,29(10):2158-2168.
• [14] 曹春雨.紫花苜蓿与蒺藜苜蓿耐盐/碱miRNA的筛选和功能鉴定 [D].重庆：重庆大学，2019.
• [15] JACOB A,LANCASTER J,BUHLER J,et al.Mercury BLASTP:accelerating protein sequence alignment [J].ACM Transactions on Reconfigurable Technology and Systems,2008,1(2):9.
• [16] KELLEY L A,MEZULIS S,YATES C M,et al.The Phyre2 web portal for protein modeling,prediction and analysis [J].Nature Protocols,2015,10(6):845-858.
• [17] OSORIO D,ARIAS M,JOHN F,et al.Peptides:a package for data mining of antimicrobial peptides [J].Bioinformatics,2015,31(9):1340-1347.
• [18] GARCION C,B??VEN L,FOISSAC X.Comparison of current methods for signal peptide prediction in phytoplasmas [J].Frontiers in Microbiology,2021,12:661524.
• [19] JONES T S,OLIVEIRA S M D,MYERS C J,et al.Genetic circuit design automation with Cello 2.0 [J].Nature Protocols,2022,17(4):1097-1113.
• [20] KRZYWINSKI M,SCHEIN J,BIROL I,et al.Circos:an information aesthetic for comparative genomics [J].Genome Research,2009,19(9):1639-1645.
• [21] 陈程杰，夏瑞.TBtools——大数据时代下的国产生物软件 [J].科学观察，2022,17(6):33-35.
• [22] WANG Y,TANG H,DEBARRY J D,et al.MCScanX:a toolkit for detection and evolutionary analysis of gene synteny and collinearity [J].Nucleic Acids Research,2012,40(7):e49.
• [23] PANDEY M,KUSHWAHA B,KUMAR R ,et al.Evol2Circos:a web-based tool for genome synteny and collinearity analysis and its visualization in fishes [J].Journal of Heredity,2020,111(5):486-490.
• [24] KUZMIN E,TAYLOR J S,BOONE C.Retention of duplicated genes in evolution [J].Trends in Genetics,2022,38:59-72.
• [25] 李满枝，龙海侠，王洪涛.基因MAFFT的多序列比对最优参数的确定 [J].基因组学与应用生物学，2016,35(7):1668-1674.
• [26] 周烺.多序列比对的可视化和特征数值化的探索与应用 [D].广州：南方医科大学，2022.
• [27] NGUYEN L T,SCHMIDT H A,VON HAESELER A.IQ-TREE:a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies [J].Molecular Biology and Evolution,2015,32(1):268-274.
• [28] VAN BEL M,SILVESTRI F,WEITZ E M,et al.PLAZA 5.0:extending the scope and power of comparative and functional genomics in plants [J].Nucleic Acids Research,2022,50(D1):D1468-D1474.
• [29] BAILEY T L,BODEN M,BUSKE F A,et al.MEME SUITE:tools for motif discovery and searching [J].Nucleic Acids Research,2009,37(Web Server issue):W202-W208.
• [30] LESCOT M,D??HAIS P,THIJS G,et al.PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences [J].Nucleic Acids Research,2002,30(1):325-327.
• [31] CARRERE S,VERDIER J,GAMAS P.MtExpress,a comprehensive and curated RNAseq-based gene expression atlas for the model legume Medicago truncatula [J].Plant and Cell Physiology,2021,62(9):1494-1500.
• [32] SALEEM M Z,BABAR Z U,SAIF S,et al.In silico characterization,physiochemical analysis,and antifungal evaluation of the Zea mays PR-1 protein [J].Scientific Reports,2025,15(1):31523.
• [33] 李波，王军，孙思.植物诱导抗病机制的研究进展 [J].中国植保导刊，2013,33(9):19-24.
• [34] KATTUPALLI D,SRINIVASAN A,SONIYA E V.A genome-wide analysis of pathogenesis-related protein-1 (PR-1) genes from Piper nigrum reveals its critical role during Phytophthora capsici infection [J].Genes (Basel),2021,12(7):1007.
• [35] VAN LOON L C,VAN STRIEN E A.The families of pathogenesis-related proteins,their activities,and comparative analysis of PR-1 type proteins [J].Physiological and Molecular Plant Pathology,1999,55(2):85-97.
• [36] ALMEIDA-SILVA F,VENANCIO T M.Pathogenesis-related protein 1 (PR-1) genes in soybean:genome-wide identification,structural analysis and expression profiling under multiple biotic and abiotic stresses [J].Gene,2022,809:146013.
• [37]PECENKOV?? T,PLESKOT R，????RSK Y V.Subcellular localization of Arabidopsis pathogenesi??s-related 1 (PR1) protein [J].International Journal of Molecular Sciences,2017,18(4):825.
• [38] FISTER A S,MEJUA L C,ZHANG A F,et al.Theobroma cacao L.pathogenesis-related gene tandem array members show diverse expression dynamics in response to pathogen colonization [J],BMC Genomics,2016,17:363.
• [39] LOTAN T,ORI N,FLUHR R.Pathogenesis-related proteins are developmentally regulated in tobacco flowers [J].The Plant Cell,1989,1(9):881-887.
• [40] AKBUDAK M A,YILDIZ S,FILIZ E,et al.Pathogenesis related protein-1 (PR-1) genes in tomato (Solanum lycopersicum L.):bioinformatics analyses and expression profiles in response to drought stress [J].Genomics,2020,112(6):4089-4099.
• [41] ZAYNAB M,PENG J,SHARIF Y,et al.Expression profiling of pathogenesis-related protein-1 (PR-1) genes from Solanum tuberosum reveals its critical role in phytophthora infestans infection [J].Microbial Pathogenesis,2021,161(Pt B):105290.